Welcome

The Molecular Biology Core Facilities (MBCF) at Dana-Farber Cancer institute (DFCI) provide expertise and access to cutting-edge genomic and proteomic technologies in a shared use setting. Although these services are primarily focused on cancer and AIDS research, most services are broadly applicable to other areas of research. We are proud to highlight some of our new services here.

Illumina NovaSeq 6000

  • High-throughput: 800M to 10B clusters/flowcell
  • Fast turnaround time for projects both small and large
  • Applications: Single-cell RNAseq, Whole Genome Sequencing, Methylation profiling…

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OpenSPR Demonstration
Thursday, March 18

Do you analyze protein-protein interactions?

… and want to confirm interactions, assemble macromolecular complexes, and perform precise kinetic analysis?

… but find that surface plasmon resonance (SPR) analysis on the BIAcore has a steep learning curve, is expensive, prone to technical issues and analysis is not intuitive?

The Proteomics facility is considering replacing the outdated BIAcore 3000 with one of the new generation of benchtop, digital, modular SPR machines that is flexible, provides equivalents of all the dominantly-used sensor chips for the BIAcore (CM5, NTA, SA, Protein A, L1 and Au) and is designed for easy, walk-up, and intuitive use with minimal training.

One of the options is the OpenSPR platform from Nicoya that will be demonstrated online by Zoom on Thursday March 18th, 2021 12pm – 1pm. Please send an email for the Zoom information if you are interested in joining us.

 

Amplicon Sequencing

  • Deep Sequencing of small amplicons (200-275bp)
  • >50,000x coverage
  • Great for CRISPR edit site characterization

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ChIPseq

  • Library prep + Sequencing from immunoprecipitated DNA
  • Fixed cost per sample
  • QC analysis and Peak Calling (optional)
  • Fast turnaround time

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Copy Number Analysis

  • Ultra Low Pass Whole Genome Sequencing (ULP-WGS)
  • 100kb resolution

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