Thursday, November 2: Cross-Site Comparison of Ribosomal Depletion Kits for Illumina RNAseq Library Construction
Tuesday, October 24, 2017: CRISPR Amplicon sequencing has been available all summer in limited batches as we worked out how to do it on robots in high throughput mode. We are now open for business. If you have 96 or only 12 to check, send us an email and we can do deep sequencing cheaply within a week. No need to clone or select primers. We do all that and give you plenty of reads per sample.
Summer 2017: Just because it isn’t listed on our website does not mean we won’t try it. We have the Oxford Nanopore minION which we will show you how to purchase and use. We’ll make the libraries and send to a PacBio for ultra-long reads. And you cannot come up with a project that we have not tried on the Illumina platforms. New Bioinformatics pipelines, Chip-Seq, RNA-Seq, expression levels from excruciatingly small amounts of starting material including single-cell. Stop on by and tell us what you want to try out.
Commonly Asked Questions
How do I collect my data?
The data distribution method depends on the specific service, whether the transfer is internal or external, and the total size of the data.
- Internal DFCI NGS data: The most fast and efficient means of data transfer is to setup a “share” folder with the help of DFCI Research Computing to allow MBCF to directly post data to your lab server immediately after sequencing and analysis are complete.
- External NGS data: We will provide a user and name and password to download data via a web browser OR we can setup post data on a secure ftp site (additional charges may apply).
- Microarray Data:
- Nanostring Data: Raw data are relatively small so we typically distribute via email
- Sanger Sequencing: Data are archived and accessible through dnaLIMS
**DATA ARE NOT DISTRIBUTED THROUGH iLAB**
How should I acknowledge the core?
- In the methods section: “(service) was performed by the Molecular Biology Core Facilities at Dana-Farber Cancer Institute.” (Please contact us if you need help writing methods for work performed by MBCF)
- If you were especially thrilled with work perform by the MBCF or you would like to acknowledge the efforts of a specific member of the core, add a line in the acknowledgements section. “We thank ______ from the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute for ____”
- If a core staff member has made a substantial contribution to the conception, design, analysis and/or interpretation of the data, authorship may be appropriate.
- If you are a Harvard University Center for AIDS Research member (CFAR), follow these directions.
Where is the MBCF located?
On the 4th floor of the DFCI Fenway campus (near the ballpark) at 21-27 Burlington Ave. Click here for directions and a map.
What is the turnaround time?
- Affymetrix Microarray: 4-6 weeks
- Nanostring: 2 weeks
- NGS library prep + sequencing: 3 weeks
- Illumina MiSeq: 1 week
- Illumina NextSeq: 2-3 weeks
*** DFCI investigators and CFAR members get priority, turnaround times may be longer for external investigators
How do I request services?
Please review the Service Request Guide for important details.
- Register an iLab account
- Place electronic iLab Request
- Drop off (clearly labeled) samples
- Await notification that results are available
Where can I drop off my samples?
There are 3 drop off locations:
1. The “Fish Room” located near the 3rd floor elevator lobby of the Smith building.
DNA: 4C refrigerator on the left
RNA: -80C freezer on the right. Please take an empty cooler from the lower half of the freezer, deposit your samples, and return the cooler + samples to the top half of the freezer to await pickup.
2. The 11th floor of the CLS building (Outside the Speigelman Lab). This is for DNA ONLY
3. Directly to the Molecular Biology Core Facilities on the 4th floor of the DFCI Fenway campus at 21-27 Burlington Ave. Click here for directions and a map.
Don’t see your question? Send us an email.