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QC + library prep + sequencing
(40M 50bp read pairs)
Peak calling + QC analysis (CHIPS)
place a request through iLab >
DNA is assayed by qubit and/or bioanalyzer to determine concentration and appropriate size distribution for Illumina sequencing (200-600bp).
Genome-wide analysis of histone modifications or TF binding.
DNA Amount: >1ng is ideal –we can work with less
DNA Size distribution: 200-600bp
Minimum Input: below detection limit of qubit and bioA
MBCF Optimal Input Amount: 2ng