ChIPseq
Chromatin immunoprecipitation sequencing (ChIPseq) is a method used to analyze DNA-protein interactions. Typical targets are histone protein modifications and transcription factors (TFs).
Pricing
QC + library prep + sequencing
(40M 50bp read pairs)
DFCI/BWH: $300
External: $360
Peak calling + QC analysis (CHIPS)
+$20/sample
Turnaround
2-3 weeks
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DNA QC
DNA is assayed by qubit and/or bioanalyzer to determine concentration and appropriate size distribution for Illumina sequencing (200-600bp).
Analytical Goals
Genome-wide analysis of histone modifications or TF binding.
Sample Requirements
DNA Amount: >1ng is ideal –we can work with less
DNA Size distribution: 200-600bp
Minimum Input: below detection limit of qubit and bioA
MBCF Optimal Input Amount: 2ng
Volume: >10ul