Molecular Biology Core Facilities Services Overview

  Updated: 12/27/2005 (Google "MBCF" for an up-to-date overview. First hit is us.)

Automated DNA Sequencing Single and double strand Dye labeled dideoxyterminators Sequence output over the network, attached e-mail, enclosed e-mail, fax or floppy Homologies, motifs, consensus sequence Submission to Genbank Current database searches Primers available Sample information Rules for editing and "Why Did My Sample Fail?"

DNA Synthesis 40 nanomole purified, quantitated 0.2 micromole 1 micromole S-oligos Biotin Aminolink Dye labeled RNA-oligos

Peptide Synthesis Amino acid analysis Analytical HPLC Preparative HPLC

Protein Sequencing Westerns Protein digests and HPLC peptide mapping Current database searches

BIAcore AB protein ligand assays User operated BIAcore instrument Ligand assays Visit BIAcore AB for overview of instrument and FAQ.

Mass Spectrometry In gel protein digests and analysis Elucidation of post translational modifications Analyze your HPLC or FPLC fractions, 100 at a time Current database digested and analyzed with fragment data Researchers are encouraged to learn how to use the instruments and analyze the data

Some of these services can only be offered to researchers currently at the Dana-Farber Cancer Institute. DNA synthesis is worldwide. Come in now and you might be oligo number 70,000 (70,000 x 26.2 average length = more than 1.8 million nucleotides synthesized).