MBCF Services Overview

Automated DNA Sequencing	DNA Synthesis	Peptide Synthesis
Protein Sequencing	Mass Spectrometry	BIAcore Ligand Assays

Automated DNA Sequencing

One thousand base runs
Dye labeled dideoxyterminators on ABI 3730 capillaries
All sequences and chromatograms in your MBCF account forever
Homologies, motifs, consensus sequence
Submission to Genbank
Current database searches
Primers available
Sample information
Rules for editing and "Why Did My Sample Fail?"
DownloadFinchTV chromatogram viewer for any computer platform. This program will allow you to view, edit, and print your DNA sequence chromatograms.

DNA Synthesis

40 nanomole purified, quantitated
S-oligos
Biotin
Aminolink
Dye labeled
RNA-oligos
MBCF list of commmercial oligo sources

Peptide Synthesis

Amino acid analysis ___See the instrument.
Analytical HPLC ___See the instrument.
Preparative HPLC ___See the instrument.

Protein Sequencing

Westerns
Protein digests and HPLC peptide mapping
Current database searches

BIAcore AB protein ligand assays

User operated BIAcore 3000 instrument
Ligand assays
Visit BIAcore ABfor overview of instrument and FAQ.
Visit the Center for Biomolecular Interaction Analysis at the University of Utah. They have software that helps to interpret Biacore data.

Mass Spectrometry

In gel protein digests and analysis
Elucidation of post translational modifications
Analyze your HPLC or FPLC fractions, 100 at a time
Current database digested and analyzed with fragment data
Researchers are encouraged to learn how to use the instruments and analyze the data

Some of these services can only be offered to researchers currently at the Dana-Farber Cancer Institute. DNA synthesis is worldwide. Come in now and you might be oligo number 70,000 (70,000 x 26.2 average length = more than 1.8 million nucleotides synthesized).