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NEW SERVICE: The Difficult Template service is recommended for those wishing to sequence hairpins, GC-rich templates, or any sample that is being "difficult" and requires special attention. Difficult Template samples require additional information that should be supplied in the comments section of the online order form.
Plasmid
50 ng/ul DNA
3.2 pmol primer already added
>12ul volume of DNA plus primer per reaction (we need at least 600ng of DNA per reaction)
PCR
5ng/ul DNA
3.2 pmol primer already added
>5ul per reaction (we need at least 25ng of DNA per reaction)
Plasmid
50 ng/ul DNA
>12ul per reaction (we need at least 600ng of DNA per reaction)
*Primer is NOT already added and either supplied in a separate tube or stored at MBCF
PCR
5ng/ul DNA
>5ul per reaction (we need at least 25ng of DNA per reaction)
*Primer is NOT already added and either supplied in a separate tube or stored at MBCF
Need a Custom Oligo? Click here to login and place an order through our Oligosyn LIMS.
PCR
5ng/ul DNA
3.2 pmol primer (either premixed or supplied separately)
>>5ul per reaction (please give us enough to repeat reaction if necessary)
BAC (must be submitted as difficult template)
1.0 ug/ul DNA (>10ul per reaction)
10uM primer (submitted separately)
*Please be sure to include additional information in the comments section of the online order form indicating why your sample is "difficult."
Questions? Email Zach or Amy or call us 617-632-3082.
